Catalase Lab Data

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Catalase Lab Data
Factors Affecting the Rate of Enzyme Activity.docx

JOVE GARCIA AND JULIE NGUYEN
Data: enzyme concentration
TRIAL
 VOLUME OF GAS PRODUCED
1 w/ Variation 1
 4.5 mL
 4.0 mL
2 w/ Variation 2
 2.5 mL
 3.0 mL
3 w/ Variation 3
 1.0 mL
 2.5 mL
4 w/ Variation 4
 2.0 mL
 1.5 mL
5 w/ Variation 5
 1.3 mL
 1.5 mL
Data: pH 7 and above (basic)
TRIAL
 VOLUME OF GAS PRODUCED
1 @ pH 8
 2.5 mL
 N/A
2 @ pH 9
 2.5 mL
 N/A
3 @ pH 10
 3.0 mL
 2.0 mL
4 @ pH 11
 2.5 mL
 N/A
5 @ pH 12
 2.5 mL
 N/A
WORD DOCUMENT VERSION
Enzyme Lab - Data (Jove & Julie).docx

Enzyme Variation
Irteza J. & Cameron E.
.
Variation
 1
 2
 3
 4
 5
Volume of enzyme (mL)
 3
 2.5
 2
 1.5
 1
Volume of water
 0
 0.50
 1
 1.5
 2
[enzyme]
 100%
 2.5/3*100=83.3%
 2/3*100= 66.7%
 1.5/3*100=50%
 1/3*100=33.3%
Height of Bubbles (First Trial)
 1 cm
 0.45 cm
 0.4 cm
 0.4cm
 0.4 cm
Volume
 3.1416 mL
d= 2cm
 1.41 mL
d= 2cm
 1.26 mL
d= 2cm
 1.26 mL
d= 2cm
 1.26 mL
d= 2cm
Height of Bubbles (Second Trial)
 1 cm
 0.4cm
 0.4 cm
 0.35 cm
 0.3 cm
Volume
 3.1416 mL
d= 2cm
 1.26 mL
d= 2cm
 1.26 mL
d= 2cm
 1.09 mL
d= 2cm
 0.942 mL
d= 2cm


pH 7 and above
Control
(No Base)
Variation 1
0.1M
Variation 2
0.01M
Variation 3
0.001M
Variation 4
0.0001M
Variation 5
0.00001M
Trial 1
6.24 mL
5.02 mL
4.40 mL
3.77 mL
3.14 mL
3.77 mL
Trial 2
4.76 mL
4.40 mL
3.14 mL
2.83 mL
2.51 mL
3.14 mL

By: Martha and Nabeelah

Temperature at 20oC and below
Trial 1
 Temperature: 20.7oC
 Amount of Gas Produced: 3.0 mL
 Enzyme Activity: 0.025 mL/s
Trial 2
 Temperature: 20.1oC
 Amount of Gas Produced: 3.0 mL
 Enzyme Activity: 0.025 mL/s
Trial 1
 Temperature: 14.5oC
 Amount of Gas Produced: 2.5 mL
 Enzyme Activity: 0.021 mL/s
Trial 1
 Temperature: 10.5oC
 Amount of Gas Produced: 0.5 mL
 Enzyme Activity: 0.0042 mL/s
Trial 1
 Temperature: 5.0oc
 Amount of Gas Produced: 2.5 mL
 Enzyme Activity: 0.021 mL/s
Trial 1
 Temperature: 1.0oC
 Amount of Gas Produced: 2.0 mL
 Enzyme Activity: 0.017 mL/s

pH 7 & above (basic)

Trial
 Volume of gas produced
Control (pH 7)
 3.5 mL
 N/A
pH 8
 3.0 mL
 N/A
pH 9
 2.25 mL
 N/A
pH 10
 3.0 mL
 4.5 mL
pH 11
 5.0 mL
 3.5 mL
pH 12
 4.0 mL
 N/A

Tahrik and Saad

Data: pH 7 and Below (acid)
Variation
 Volume of Gas Produced
Trial 1
 Trial 2
1 neutral
 3.8mL
 N/A
2 pH2
 4.2mL
 N/A
3 pH3
 4.7mL
 N/A
4 pH4
 2.3mL
 4.1mL
5 pH5
 4.2mL
 N/A
6 pH6
 3.2mL
 N/A

Data: Enzyme Concentration
Variation
 Volume of Gas Produced
Trial 1
 Trial 2
1 3mL enzyme
 6.4mL
 5.2mL
2 2.5mL enzyme + 0.5mLwater
 2.4mL
 2.6mL
3 2.0mL enzyme + 1.0mL water
 2.1mL
 N/A
4 1.5mL enzyme + 1.5mL water
 1.5mL
 N/A
5 1.0mL enzyme + 2.0mL water
 0.4mL
 N/A
Rajavi and Alyssa

Substrate Concentration
Variation
 Volume of Bubbles (Trial 1)
1. 100%
 4.5mL
2. 80%
 3mL
3. 60%
 2mL
4. 40%
 1.5mL
5. 20%
 1.2mL

pH 7 and below (acidic)
pH variation
 Volume of Bubbles (Trial 1)
Neutral
 4mL
pH 6
 3.5mL
pH 5
 3mL
pH 4
 2mL
pH 3
 3mL
pH 2
 3.5mL

Sarjini & Annie


Procedure 6: pH 7 and above (basic)
*Radius was 1 cm
pH Level
Volume of Foam (mL)
Trial 1
 Trail 2
7 (control)
7.2
 5.96
8
 6.6
 N/A
9
 5.7
 N/A
10
 5.7
 N/A
11
 5.3
 N/A
12
 5.0

 N/A


Procedure 1: Temperature 20 degrees Celsius and above
Trial
 Temperature (degrees Celsius)
 Volume of foam (mL)
1
 23
 6.8
2
 23
 5.338
1
 25
 5.966
2
 25
 N/A
1
 30
 6.8
2
 30
 N/A
1
 35
 7.222
2
 35
 N/A
1
 40
 9.106
2
 40
 N/A

Nathan and Aynul

Substrate Concentration

Variation
 volume of gas produced
Substrate concentration
 Trial 1
 Trial 2
100%
 4.5mL
 3mL
80%
 2.5mL
 3mL
60%
 3mL
 2.5mL
40%
 1.5mL
 2mL
20%
 0.5mL
 0.5mL

pH 7 and above (base)


Variation
 volume of gas produced
pH
 Trial 1
 Trial 2
7
 4mL
 3.5mL
8
 2.5mL
 3mL
9
 2.5mL
 2.5mL
10
 1mL
 3mL
11
 1.5mL
 3mL
12
 3mL
 2mL




Nejaha and Anne
pH below (Acidic)
Trial
 Amount of gas
1
 pH 2 1.5 mL
2
 pH 2 2 mL
1
 pH 3 N/A
2
 pH 3 2 mL
1
 pH 4 2.5mL
2
 pH 4 2 mL
1
 pH 5 2 mL
2
 pH 5 3 mL
1
 pH 6 2.5mL
1
 pH 7 5 mL
Temperature 20 C and above
Temperature
 Trial
 Amount of foam
20 C
 1
 2.8 mL
27 C
 1
 3.8 mL
33 C
 1
 6.3 mL
38 C
 1
 7.8 mL
42 C
 1
 7.8 mL




Joey and Alexis

Substrate Concentration
Variation
 Volume of Gas Produced
1 - 100%
 6mL of bubbles
2 - 80%
 6.5mL of bubbles
3 - 60%
 1mL of bubbles
4 - 40%
 1.75mL of bubbles
5 - 20%
 1mL of bubbles

pH Below (Acidity)

pH Varitation
 Volume of Gas Produced
2
 4.5mL of bubbles
3
 4mL of bubbles
4
 3mL of bubbles
5
 2.5mL of bubbles
6
 4mL of bubbles
7 (neutral)
 6.5mL of bubbles

KATHRYN & SHANUKHA
Procedure Two: Temperature below 20 Degrees Celsius

Trial
Before
During
After
1 (@ 23 degrees)
Enzyme: Translucent, brown, liquid, some suspension
Substrate: Clear, transparent liquid
 White foam forming on top of a beige, opaque liquid.
23mL Foam high formed
2 (@ 15 degrees)
 No Change
 No change
 17mL Foam high formed
3 (@ 10 degrees)
 No Change
 No change
 4mL Foam high formed
4 (@ 6 degrees)
 No Change
 No change
 3.5mL Foam high formed
5 (@ 1 degrees)
 No Change
 No change
 10mL Foam high formed (first time) 6mL of foam high formed (second time)

Procedure Five: pH below 7
Trial
Before
During
After
1 (pH 7)
Enzyme: Translucent, brown, liquid, some suspension
Substrate: Clear, transparent liquid
 White foam forming on top of a beige, opaque liquid.
18mL Foam high formed
2 (HCl @ 10^-5M)
 No Change
 No change
 6mL Foam high formed
3 (HCl @ 10^-4M)
 No Change
 No change
 15mL Foam high formed
4 (HCl @ 10^-3M)
 No Change
 No change
 13mL Foam high formed
5 (HCl @ 0.01M)
 No Change
 No change
 16mL Foam high formed
6 (HCl @ 0.10M)
 No Change
 No change
 14mL Foam high formed